Diversity of partial sequence leptin gene (Exon 3) in crossbred cattle compared to GenBank database

Abstract. Ermawati D, Panjono, Bintara S, Hartatik T. 2022. Diversity of partial sequence leptin gene (Exon 3) in crossbred cattle compared to GenBank database. Biodiversitas 23: 5614-5620. Genetic marker method that is often used select Single Polymorphism Nucleotide (SNP). Polymorphisms (SNP) has been found various candidate genes, one which the gene. Leptin a affects reproduction and weight. Therefore, this research aims identify SNP (exon Crossbred by comparison with data. A sample was taken from Klaten Central Java, Indonesia. Afterward, DNA sequencing results were analyzed BioEdit SNP. software for alignment part intron 2, exon 3 3'UTR. The MEGA 11.1.1 program analyze edit genetic distance phylogenetic tree based on result shows nine variations SNPs 3. cattle, Bos taurus, indicus, Hereford, Friesian Holstein Bali, same cluster. HindIII restriction enzyme can be recommended genotyping using PCR RFLP. This provides information so it further association growth reproduction.